Fingerprinting and Determination of the Content of Wuwei Ganlu Yaoyu Decoction Powder (五味甘露药浴汤散) of Zang Medicine

Author：YANG Junfeng1,2,3, WU Haozhen1,2,3, WU Minghui1,2,3, BAIMA Danzeng3,4, ZHU Yejing5, XIE Hebing1,2,3,4

Unit：1.School of Pharmacy, Anhui University of Chinese Medicine, Hefei Anhui 230012, China; 2.Yangtze Delta Drug Advanced Research Institute, Nantong Jiangsu 226133, China; 3.Jiangsu Shenhou Pharmaceutical Research Co, Ltd, Nantong Jiangsu 226133, China; 4.Xizang Shenhou Pharmaceutical Co., Ltd, Xigaze Xizang 857000, China; 5.School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing Jiangsu 210023, China

Quote：引用：杨俊峰，吴浩震，吴明慧，白玛旦增，朱业锦，谢和兵.藏药五味甘露药浴汤散指纹图谱及指标成分含量测定研究[J].中医药导报,2025,31(1):67-70,76.

DOI：10.13862/j.cn43-1446/r.2025.01.012

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Abstract：

Objective: To establish the high performance liquid chromatographic (HPLC) fingerprints of Wuwei Ganluyaoyu decoction powder, and determine the contents of flavonoid index components, including hyperoside, luteoloside, quercitrin, quercetin and amentoflavone. Methods: The separation was performed on an Ultimate XB-C18 column (250.0 mm×4.6 mm，5.0 μm) with the mobile phase of acetonitrile-0.4% phosphoric acid solution at a flow rate of 1 mL/min and a detection wavelength of 360 nm. The column temperature was 30 ℃, with the injection volume of 10 μL. Results: The HPLC fingerprints of Wuwei Ganluyaoyu decoction powder identified a total of 16 common peaks, and the similarity of the fingerprints was >0.99. Hyperoside, luteoloside, quercitrin, quercetin and amentoflavone were found in the ranges of 4.91-98.10 μg/mL(r=1.000 0), 4.93-98.60 μg/mL (r=0.999 6)，4.99-99.92 μg/mL (r=1.000 0), 2.50-49.80 μg/mL (r=0.999 9) and 2.48-49.50 μg/mL (r=0.999 8) with good linearity and the average recoveries (n=6) were 98.0% (RSD=0.52%), 96.3% (RSD=1.79%), 100.2% (RSD=0.54%), 100.4% (RSD=0.70%), 99.6% (RSD=0.80%), respectively. Conclusion: The established fingerprints and the multi-component content determination method are highly specific, precise and stable, which can provide a basis for improving the quality standard of Wuwei Ganluyaoyu decoction powder.

Key words：Wuwei Ganluyaoyu decoction powder; Zang medicine; HPLC; fingerprinting; quality evaluation

摘要：

目的：建立藏药五味甘露药浴汤散高效液相色谱（HPLC）法指纹图谱，并测定黄酮指标成分金丝桃苷、木犀草苷、槲皮苷、槲皮素和穗花杉双黄酮的含量。方法：采用Ultimate XB-C18（250.0 mm×4.6 mm，5.0 μm)色谱柱；流动相为乙腈-0.4%磷酸溶液；梯度洗脱；流速为1 mL/min；检测波长为360 nm；柱温为30 ℃；进样量为10 μL。结果：五味甘露药浴汤散HPLC指纹图谱共标识出16个共有峰，指纹图谱相似度＞0.99。黄酮指标成分金丝桃苷、木犀草苷、槲皮苷、槲皮素、穗花杉双黄酮分别在4.91~98.10 μg/mL（r=1.000 0）、4.93~98.60 μg/mL（r=0.999 6）、4.99~99.92 μg/mL（r=1.000 0）、2.50~49.80 μg/mL（r=0.999 9）、2.48~49.50 μg/mL（r=0.999 8）范围内线性关系良好，平均加样回收率（n=6）分别为98.0%（RSD=0.52%）、96.3%（RSD=1.79%）、100.2%（RSD=0.54%）、100.4%（RSD=0.70%）、99.6%（RSD=0.80%）。结论：建立的指纹图谱及多成分含量测定方法专属性强、精密度高、稳定性好，可为提升五味甘露药浴汤散的质量标准提供依据。

关键词：五味甘露药浴汤散；藏药；HPLC；指纹图谱；质量评价

Release time：2025-11-28

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