Optimization of the Extraction Process of Quercetin from Alpinia officinarum Hance by Orthogonal Experiment Method

Author：LIN Wenxin1, HUANG Junwei1, LI Ruyi1, RUAN Qingling2, YANG Canfeng2, NIE Yuwen2, FENG Zhenying3

Unit：1.Guangzhou Skin Disease Prevention and Treatment Institute, Guangzhou Guangdong 510095, China; 2.Lingnan Normal University, Zhanjiang Guangdong 524048, China; 3.The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang Guangdong 524001, China

Quote：引用：林文新，黄俊炜，李儒亿，阮清玲，杨灿凤，聂雨雯，冯真英.正交试验法优化高良姜中槲皮素的提取工艺研究[J].中医药导报,2025,31(12):106-109,120.

DOI：10.13862/j.cn43-1446/r.2025.12.016

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Abstract：

Objective: To optimize the ultrasonic-assisted extraction conditions for quercetin from Gaoliangjiang (Alpinia officinarum Hance). Methods: Based on single-factor experiments, quercetin was quantitatively analyzed by high-performance liquid chromatography (HPLC). The effects of ethanol concentration, ultrasonic time, and solid-liquid ratio on quercetin content were screened using an orthogonal experiment. Results: The optimal extraction conditions were as follows: solid-liquid ratio of 1∶18 (g/mL), ultrasonic time of 50 min (power 400 W, frequency 25 Hz), and ethanol concentration of 75%. Under these conditions, the extraction yield of quercetin from Alpinia officinarum Hance was 88.15 μg/g. Conclusion: The optimized ultrasonic extraction process for quercetin from Alpinia officinarum Hance is reasonable, stable, and feasible, providing a reference for the extraction of quercetin from this herb.

Key words：Alpinia officinarum Hance; quercetin; single-factor investigation; orthogonal test; ultrasonic extraction

摘要：目的：探讨超声辅助优化高良姜中槲皮素的提取工艺条件。方法：在单因素试验的基础上，采用高效液相色谱法定量分析槲皮素，通过正交试验筛选乙醇体积分数、超声时间及料液比对槲皮素含量的影响。结果：高良姜中槲皮素的最佳提取工艺条件为料液比1∶18（g/mL），超声时间（功率400 W，频率25 Hz）50 min，乙醇体积分数75%。在该工艺条件下，高良姜中槲皮素的提取量为88.15 μg/g。结论：优选的高良姜中槲皮素超声提取工艺合理、稳定、可行，可为高良姜中槲皮素的提取提供参考。

关键词：高良姜；槲皮素；单因素考察；正交试验；超声提取

Release time：2026-01-30

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