Mechanism of Gusong Kangfu Decoction (骨松康复汤) against Osteoporosis via Network Pharmacology, Molecular Docking, and Animal Experiments

Author：JI Futao1, LU Yu2, XIE Yuanyang3, REN Silu3, YIN Benjing2

Unit：1.Zhengzhou 460 Hospital, Zhengzhou He'nan 450007 China; 2.Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming Yunnan 650021,China; 3.Yunnan University of Traditional Chinese Medicine, Kunming Yunnan 650500, China

Quote：引用：吉富涛，卢钰，谢元洋，任思路，尹本敬.基于网络药理学、分子对接和动物实验探究骨松康复汤抗骨质疏松症的作用机制[J].中医药导报,2026,32(4):5-12.

DOI：10.13862/j.cn43-1446/r.2026.04.002

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Abstract：

Objective: To elucidate the molecular mechanism of Gusong Kangfu decoction against osteoporosis (OP) by integrating network pharmacology, molecular docking, and animal experiments. Methods: Active ingredients and targets of Gusong Kangfu decoction were screened using the TCMSP database. OP-related targets were retrieved from GeneCards, OMIM, TTD, DrugBank, and DisGeNET databases. Potential targets were identified by intersecting drug and disease targets. A protein-protein interaction (PPI) network was constructed via STRING to identify core targets. Functional enrichment analysis was performed with DAVID, and molecular docking was conducted using AutoDock 4.2.6. An OP mouse model was established by bilateral ovariectomy. Totally 24 mice were randomly assigned to sham group, model group, estrogen group, and Gusong Kangfu decoction group. After 12 weeks of treatment, femurs were harvested for validation of key targets and pathways. Results:  Totally 319 active ingredients and 448 targets of Gusong Kangfu decoction were identified, with 293 potential targets overlapping with OP-related targets. KEGG enrichment analysis revealed significant involvement of targets in the phosphoinositide 3-kinase (PI3K)-protein kinase B (Akt) signaling pathway. PPI network analysis identified nuclear factor kappa B inhibitor alpha (NFKBIα), fos proto-oncogene (FOS), hypoxia inducible factor 1 subunit alpha (HIF-1α), catenin beta 1 (CTNNB1), and mitogen-activated protein kinase 14 (MAPK14) as core targets. Molecular docking confirmed strong binding affinities (binding energy＜-7.0 kcal/mol) between five key components (quercetin, wogonin, luteolin, stigmasterol, kaempferol) and these core targets. Animal experiments demonstrated that Gusong Kangfu decoction significantly down-regulated expression of NFKBIA mRNA, FOS mRNA, HIF-1α mRNA, and MAPK14 mRNA (P＜0.05), while upregulating CTNNB1 mRNA and p-PI3K/p-Akt protein levels (P＜0.05) in femoral tissue. Conclusion: Gusong Kangfu decoction targets NFKBIα, FOS, HIF-1α, MAPK14 and CTNNB1 targets through quercetin and other ingredients to activate the PI3K-Akt signaling pathway, which then exerts anti-OP effects.

Key words：osteoporosis; Gusong Kangfu decoction; network pharmacology; molecular docking; experimental validation; mouse

摘要：

目的：整合网络药理学、分子对接及动物实验揭示骨松康复汤抗骨质疏松症（OP）的分子机制。方法：通过TCMSP筛选骨松康复汤活性成分及靶点；从GeneCards、OMIM、TTD、DrugBank及DisGeNET数据库获取OP疾病靶点，取交集得潜在靶点。利用STRING构建蛋白质-蛋白质相互作用（PPI）网络筛选核心靶点，DAVID进行通路富集分析，AutoDock 4.2.6完成分子对接。采用双侧卵巢切除术建立OP小鼠模型，将24只小鼠随机分为假手术组、模型组、雌激素组和骨松康复汤组，治疗12周后取股骨验证靶点及通路。结果：鉴定骨松康复汤活性成分319个、靶点448个，与OP靶点交集得293个潜在靶点。KEGG分析显示靶点显著富集于磷脂酰肌醇3激酶（PI3K）-蛋白激酶B（Akt）等通路。PPI网络确定NF-κB抑制蛋白α（NFKBIα）、Fos原癌基因（FOS）、缺氧诱导因子1α（HIF-1α）、连环蛋白β1（CTNNB1）和丝裂原活化蛋白激酶14（MAPK14）为核心靶点。分子对接证实槲皮素、汉黄芩素、木犀草素、豆甾醇、山柰酚与核心靶点结合能均＜-7.0 kcal/mol。动物实验显示：骨松康复汤能显著下调股骨NFKBIA mRNA、FOS mRNA、HIF-1α mRNA和MAPK14 mRNA表达（P＜0.05），上调CTNNB1 mRNA及p-PI3K/p-Akt蛋白水平（P＜0.05）。结论：骨松康复汤可通过槲皮素等活性成分靶向调控NFKBIA、FOS、HIF-1α、MAPK14和CTNNB1，激活PI3K-Akt信号通路发挥抗OP作用。

关键词：骨质疏松症；骨松康复汤；网络药理学；分子对接；实验验证；小鼠

Release time：2026-04-22

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