Fuhu Qushi Formula (茯虎祛湿方) Ameliorates Eczema in Mice by Inhibiting the FGFR4/NF-κB Signaling Pathway

Author：CHENG Lu1, KANG Jianying1, YAN Lishan1, GU Chunyu1, HUANG Zhishan1, LI Jiajia1, TAN Qiling1, LIU Wenling1, QIU Xinyu1, JIA Zhanhong1, HAN Bing2, ZHANG Yi1

Unit：1.Beijing University of Chinese Medicine, Beijing 100102, China; 2.Heilongjiang Jiren Pharmaceutical Co., Ltd., Harbin Heilongjiang 150000, China

Quote：引用：成璐，康建英，颜丽珊，顾春宇，黄芷珊，李嘉佳，谭淇玲，刘文玲，邱新宇，贾占红，韩冰，张翼.茯虎祛湿方通过抑制FGFR4/NF-κB信号通路改善小鼠湿疹的作用研究[J].中医药导报,2026,32(2):36-46.

DOI：10.13862/j.cn43-1446/r.2026.02.007

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Abstract：

Objective: To investigate the therapeutic effect of Fuhu Qushi formula (FH) on acute and chronic eczema mouse models induced by 2,4-dinitrochlorobenzene (DNCB). Methods: The chemical constituents of FH were analyzed and identified using ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Acute and chronic eczema mouse models were established by stimulation with DNCB. Scratching episodes and ear swelling in the eczema mice were recorded. Hematoxylin-eosin (HE) staining was used to observe histopathological changes in the ear tissue of chronic eczema mice. Serum levels of interleukin-1β (IL-1β) and immunoglobulin E (IgE) in chronic eczema mice were detected by enzyme-linked immunosorbent assay (ELISA). The expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), p-p65, p-p38, and FGFR4 in the ear tissue was detected by immunohistochemistry. Molecular docking was used to validate the affinity between the top five chemical components (based on response ranking) and FGFR4. Results: A total of 96 chemical components were identified in FH, including paeonol, dictamnine, fraxinellone, polydatin, baicalin, rutin, quercetin, mudanpioside E, paeoniflorin, and galloylpaeoniflorin. Compared with the control group, the model group showed significantly increased scratching episodes and more severe ear swelling. In the chronic eczema model group, the epidermis exhibited hyperkeratosis or parakeratosis, thickening of the granular and prickle cell layers, dermal edema, and inflammatory cell infiltration. Serum levels of IL-1β and IgE were elevated (P<0.05), and the expression of TNF-α, IL-6, TLR4, p-p65, and p-p38 in ear tissue was increased in the chronic eczema model group (P<0.05). Compared with the model group, FH administration significantly reduced scratching episodes and alleviated ear swelling in both acute and chronic eczema mice, improved histopathological manifestations, and decreased serum levels of IL-1β and IgE (P<0.05). Molecular docking results showed good binding affinity between the main components of FH and FGFR4. Immunohistochemical results showed that FH significantly inhibited the expression of FGFR4, p-p65, p-p38, TNF-α, and IL-6 in the ear tissue of chronic eczema mice. Conclusion: Fuhu Qushi formula can inhibit the inflammatory response in DNCB-induced eczema model mice, and the mechanism is related to the inhibition of the FGFR4/NF-κB signaling pathway.

Key words：eczema; Fuhu Qushi formula; component analysis; FGFR4/NF-κB signaling pathway

摘要：

目的：探究茯虎祛湿方（FH）对2,4-二硝基氯苯（DNCB）诱导的急、慢性湿疹模型小鼠的治疗作用。方法：采用超高效液相色谱-四极杆-飞行时间串联质谱（UPLC-Q-TOF-MS/MS）技术对FH化学成分进行分析鉴定；建立DNCB刺激诱导的急、慢性湿疹小鼠模型，观测湿疹小鼠瘙痒次数及其耳肿胀度；采用苏木素-伊红（HE）染色法观察慢性湿疹小鼠耳组织的病理变化；采用酶联免疫吸附试验（ELISA）检测慢性湿疹小鼠血清IL-1β和IgE的水平；采用免疫组织化学染色法检测慢性湿疹小鼠耳组织TNF-α、IL-6、p-p65、p-p38和FGFR4表达；应用分子对接技术对响应排名前5的化学成分与FGFR4之间的亲和力进行验证。结果：FH中含有丹皮酚、白鲜碱、梣酮、虎杖苷、黄芩苷、芦丁、槲皮素、牡丹皮苷E、芍药苷、没食子酰芍药苷等96种化学成分。与对照组比较，模型组小鼠瘙痒次数显著增加，耳肿胀明显；慢性湿疹模型组小鼠表皮层出现角化过度或角化不全，颗粒层及棘层肥厚，真皮层水肿及炎症细胞浸润，血清中IL-1β和IgE水平升高（P＜0.05），且耳组织中TNF-α、IL-6、TLR4、p-p65和p-p38的表达增加（P＜0.05）。与模型组比较，FH给药能减少急、慢性湿疹小鼠瘙痒次数，减轻其耳肿胀情况，改善慢性湿疹小鼠病理表现，降低其血清中IL-1β和IgE水平（P＜0.05）。分子对接结果显示，FH中主要成分与FGFR4具有较好的亲和力。免疫组化结果显示，FH能抑制慢性湿疹小鼠耳组织FGFR4、p-p65、p-p38、TNF-α和IL-6的表达。结论：FH能抑制DNCB诱导的湿疹模型小鼠的炎症反应，其机制与抑制FGFR4/NF-κB信号通路相关。

关键词：湿疹；茯虎祛湿方；成分分析；FGFR4/NF-κB信号通路

Release time：2026-03-05

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